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Journal: Science Advances
Article Title: Copy-scAT: Deconvoluting single-cell chromatin accessibility of genetic subclones in cancer
doi: 10.1126/sciadv.abg6045
Figure Lengend Snippet: ( A ) Banked cryopreseved aGBM samples were used for both scATAC and WGS. Nuclei were isolated from the samples, mixed, and used for both scATAC and WGS. ( B to D ) Percent of chromosome arm-level gains, chromosome arm-level losses, and focal amplifications detected in aGBM samples identified using both methods versus CNVs detected by scATAC or WGS alone. ( E ) Banked frozen pGBM samples were used for both scATAC and WGS. ( F and G ) Number of chromosome arm-level gains and losses detected in pGBM samples identified using both methods versus total numbers of gains detected by scATAC or WGS. ( H ) Fresh MM samples were used for both scATAC and 10x scCNV DNA analysis. ( I and J ) Number of chromosome arm-level gains and losses detected by both methods versus total numbers of gains detected by scATAC or 10x scCNV analysis. ( K ) Overview of raw transformed z -score profiles for four aGBM samples.
Article Snippet: Third, we benchmarked Copy-scAT against CNV calls made with the
Techniques: Isolation, Transformation Assay